| 产品编号 | bs-46004P |
| 英文名称 | Recombinant SARS-Cov-2 Spike S1 protein, His & Avi (HEK293) |
| 中文名称 | 重组SARS冠状病毒2 Spike S1 蛋白 |
| 别 名 | coronavirus spike Protein; cov spike Protein; novel coronavirus RBD Protein; novel coronavirus s1 Protein; novel coronavirus spike Protein; RBD Protein; S1 Protein; Spike RBD Protein; 2019-nCoV; 2019nCoV; Recombinant SARS-Cov-2 Spike S1 protein, His & Avi (HEK293) 新冠病毒S蛋白; 新冠S1蛋白; SARS-CoV-2 S1蛋白; |
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Specific References (1) | bs-46004P has been referenced in 1 publications.
[IF=4.38] Lalioti, Vasiliki. et al. Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia. SCI REP-UK. 2022 Apr;12(1):1-19 Other ; Other.
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| 克 隆 号 | |
| 理论分子量 | 73 kDa |
| 检测分子量 | 120-140 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | SARS-Cov-2 |
| 序 列 | 14-683/1273 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | Not analyzed |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | His & Avi |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in 2X PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| 产品图片 |
Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: bs-46001P) at 2μg/ml (100ul/Well) can bind SARS-CoV-2 Spike S1, His-Avi Tag (Cat: bs-46004P), the EC50 is 912.27ng/ml.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
