| 产品编号 | bsm-41412M |
| 英文名称 | SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse mAb |
| 中文名称 | SARS冠状病毒2核衣壳蛋白单克隆抗体 |
| 别 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein. |
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Specific References (2) | bsm-41412M has been referenced in 2 publications.
[IF=8.116] Valeria Napolitano. et al. Acriflavine, a clinically approved drug, inhibits SARS-CoV-2 and other betacoronaviruses. Cell Chem Biol. 2022 Jan;: IF ; Human.
[IF=4.123] Chunyan Zhang. et al. Foundation and Clinical Evaluation of a New Method for Detecting SARS-CoV-2 Antigen by Fluorescent Microsphere Immunochromatography. Front Cell Infect Mi. 2020; 10: 553837
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| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 7A7 |
| 交叉反应 | (predicted: SARS-CoV-2) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | ≥1 mg/ml |
| 免 疫 原 | Recombinant SARS-CoV-2 Nucleocapsid Protei: full length |
| 亚 型 | IgG2b |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 产品图片 |
Sample:
Lane 1: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng
Lane 2: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng
Lane 3: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng
Lane 4: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng
Lane 5: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng
Lane 6: Recombinant SARS-CoV-2 N Protein(WT) at 10ng
Primary: Mouse Anti- SARS-CoV-2 N protein (bsm-41412M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size:45.3 kD
Observed band size:50 kD
Sample:
Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng
Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng
Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng
Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng
Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng
Lane 6:SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng
Primary: Anti- SARS-CoV-2 N protein (bs-41412M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Sample:
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P)
Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P)
Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P)
Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P)
Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P)
Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P)
Primary: Anti-SARS-CoV-2(2019-nCoV)Nucleocapsid(bsm-41412M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
