| 产品编号 | bsm-49135M |
| 英文名称 | SARS-CoV Nucleocapsid Mouse mAb |
| 中文名称 | SARS病毒核衣壳蛋白单克隆抗体 |
| 别 名 | N [SARS coronavirus ShanghaiQXC2]; |
| 研究领域 | 细菌及病毒 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 7C2B |
| 交叉反应 | (predicted: SARS) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | ≥1mg/ml |
| 免 疫 原 | Recombinant SARS N (nucleocapsid): 1-422/422aa |
| 亚 型 | IgG2b |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. The nucleocapsid (N) protein of SARS-CoV enters the host cell together with the viral RNA and interferes with several cellular processes. Some of these processes involve interactions between SARS-CoV N protein and host-cell proteins. It has also been demonstrated that the SARS-CoV N protein can bind to DNA in vitro. These interactions might have a role in the pathology of SARS. The N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS. |
| 产品图片 |
Sample:
Lane 1: 2019-nCoV Nucleocapsid Protein full length (His tag) Overexpression E.coli Lysate (Cat#: bs-41408P) at 30 ug
Lane 2: Recombinant SARS N (1-422) protein (Cat#: bs-49002P) at 5 ug
Primary: Anti-SARS N (nucleocapsid) (bsm-49135M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Sample:
Lane 1: SARS-CoV-2 N Protein (Cat#: NP) at 4 ug
Lane 2: Recombinant SARS N (1-422) protein (Cat#: bs-49002P) at 5 ug
Lane 3: MERS N protein (Cat#: bs-40068-V08B) at 2 ug
Primary: Anti-SARS N (nucleocapsid) (bsm-49135M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
