| 产品编号 | bs-41450P |
| 英文名称 | Recombinant SARS-Cov-2 Spike RBD protein (Y453F), His |
| 中文名称 | 重组SARS冠状病毒2 Spike突变蛋白RBD (Y453F) |
| 别 名 | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2; 2019-nCOV Spike RBD (Y453F), Recombinant (His Tag); Recombinant SARS-Cov-2 Spike RBD protein (Y453F), His 突变型新冠蛋白; 新冠病毒S蛋白突变体（Y453F）; 重组新冠病毒 Spike突变蛋白RBD(Y453F); 新型冠状病毒Spike突变体蛋白; |
| 克 隆 号 | |
| 理论分子量 | 27.8 kDa |
| 检测分子量 | 36 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | SARS-Cov-2 |
| 序 列 | 319-541/1273 |
| 纯 度 | >90% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | Not analyzed |
| 表达系统 | E.coli |
| 活性 | Yes |
| 标签 | His |
| 缓 冲 液 | PBS (pH7.4). |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to specific receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be necessary for the binding of the virus to the host cell at the advent of the infection process. It has been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor-binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 provides essential elements needed for membrane fusion. The S protein plays vital parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; the Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor-binding domain (RBD), leading to a higher mortality rate.
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| 产品图片 |
Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: bs-46001P) at 2μg/ml (100ul/Well) can bind SARS-CoV-2 Spike RBD (Y453F), His Tag (Cat: bs-41450P) , the EC50 is 8.34ng/ml.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
