| 产品编号 | bs-40295G-HRP |
| 英文名称 | AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记亲和纯化羊抗兔IgG (H+L)(进口分装) |
| 别 名 | AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)/HRP;
AffiniPure Goat Anti-Rabbit IgG H&L/HRP;
AffiniPure Goat Anti-Rabbit IgG H&L(HRP);
HRP AffiniPure Goat Anti-Rabbit IgG (H+L);
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) HRP标记山羊抗兔IgG (H+L); 辣根过氧化物酶标记羊抗兔IgG (H+L)(进口分装); |
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Specific References (59) | bs-40295G-HRP has been referenced in 59 publications.
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| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rabbit |
| 产品应用 | WB=1:5000-20000,IHC-P=1:3000-5000,IHC-F=1:3000-5000,ELISA=1:5000-20000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1.0 mg/ml |
| 免 疫 原 | Native Rabbit IgG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others. Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications. |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin polyclonal antibody, unconjugated (bs-0061R) at 1:10000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody (bs-40295G-HRP) at 1:20000 dilution and r.t. for 60 min incubation.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
