| 产品编号 | bsm-33313M |
| 英文名称 | Gamma Tubulin Mouse mAb |
| 中文名称 | 微管蛋白γ单克隆抗体 |
| 别 名 | Centrosome Marker; gamma 1 tubulin; gamma tubulin; gamma-tubulin; gamma 2 tubulin; gamma Tubulin 1; gamma Tubulin 2; gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; gamma-1-tubulin; gamma-tubulin complex component 1. |
| 研究领域 | 细胞生物 神经生物学 细胞骨架 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 8D11 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-5000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Gamma Tubulin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Centrosome Marker Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Function: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Subunit: Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Tissue Specificity: Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. Post-translational modifications: Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. Similarity: Belongs to the tubulin family. SWISS: Q9NRH3 Gene ID: 7283 Database links: Entrez Gene: 27175 Human Entrez Gene: 7283 Human Entrez Gene: 103733 Mouse Entrez Gene: 103768 Mouse Omim: 191135 Human Omim: 605785 Human SwissProt: P23258 Human SwissProt: Q9NRH3 Human SwissProt: P83887 Mouse SwissProt: Q8VCK3 Mouse Unigene: 279669 Human Unigene: 708059 Human Unigene: 142348 Mouse Unigene: 479145 Mouse Unigene: 154431 Rat 结构蛋白（Structural Proteins） gamma tubulin 微管蛋白是存于中心体的另一种微管蛋白, gamma微管蛋白对微管的形成具有重要作用。gamma微管蛋白通过与β-微管蛋白的相互作用帮助微管的成核反应(nucleation)。即在微管的组装中gamma微管蛋白先形成一个圆或形成钩环结构, γ微管蛋白的这种结构可指导微管蛋白二聚体结合上去并进行微管的组装。gamma微管蛋白在微管的组装中起关键作用。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Gamma Tubulin monoclonal antibody, unconjugated (bsm-33313M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control（black line）:Hela.
Primary Antibody (green line):Mouse Anti-Gamma Tubulin antibody (bsm-33313M)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
