产品编号	bsm-33040M
英文名称	lamin B1 Mouse mAb, Nuclear Loading Control
中文名称	核纤层蛋白B（核内参）单克隆抗体
别名	LMNB1_HUMAN; Lamin-B1; LMNB1; LMN2; LMNB; LMN; ADLD; MCPH26;
	Specific References (1) \| bsm-33040M has been referenced in 1 publications. [IF=5.195] Yanqiu Sun. et al. Xinfeng capsule inhibits inflammation and oxidative stress in rheumatoid arthritis by up-regulating LINC00638 and activating Nrf2/HO-1 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115839 WB ; Human. PubMed:36272490
产品类型	内参抗体
研究领域	细胞生物染色质和核信号信号转导细胞凋亡细胞类型标志物
抗体来源	Mouse
克隆类型	Monoclonal
克隆号	9C11
交叉反应	Human,Rat
产品应用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	64 kDa
检测分子量
细胞定位	细胞核
性状	Liquid
浓度	1mg/ml
免疫原	Recombinant human Lamin B Protein: 314-386/586
亚型	IgG1
纯化方法	affinity purified by Protein G
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]. Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin Subunit: Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Subcellular Location: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side Post-translational modifications: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. DISEASE: Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. Similarity: Belongs to the intermediate filament family. SWISS: P20700 Gene ID: 4001 Database links: Entrez Gene: 396223 Chicken Entrez Gene: 4001 Human Entrez Gene: 16906 Mouse Entrez Gene: 116685 Rat Omim: 150340 Human SwissProt: P14731 Chicken SwissProt: P20700 Human SwissProt: P14733 Mouse SwissProt: P70615 Rat Unigene: 89497 Human Unigene: 4105 Mouse Unigene: 11362 Rat
产品图片	Sample: Lane 1: Human Molt-4 cell lysates Lane 2: Human Hela cell lysates Lane 3: Human U-2os cell ysates Primary: Anti-Lamin B (bsm-33040M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa Sample: Lane 1: Rat Pancreas tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Rat Thymus tissue lysates Lane 4: Human Jurkat cell lysates Lane 5: Human Molt-4 cell lysates Lane 6: Human Hela cell lysates Lane 7: Human U-2os cell ysates Lane 8: Human THP-1 cell lysates Primary: Anti-Lamin B (bsm-33040M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of bsm-33040M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (bsm-33040M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序