| 产品编号 | bsm-33128M |
| 英文名称 | Plant actin Mouse mAb, Loading Control |
| 中文名称 | 植物肌动蛋白（内参）单克隆抗体 |
| 别 名 | Plant-actin; beta Actin; 植物内参; 植物肌动蛋白内参; |
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Specific References (1) | bsm-33128M has been referenced in 1 publications.
[IF=6.064] Jianan Hu. et al. Identification of Key Residues Essential for the Activation of Plant Immunity by Subtilisin From Bacillus velezensis LJ02.. FRONT MICROBIOL. 2022 Aug;13:869596-869596 WB ; Nicotiana benthamiana.
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| 产品类型 | 内参抗体 植物抗体 |
| 研究领域 | 细胞生物 发育生物学 植物 细胞骨架 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 4F5 |
| 交叉反应 | Various Plants |
| 产品应用 | WB=1:2000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Synthetic MAP peptide derived from human beta-Actin |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting Database links: SwissProt: P0CJ46 ARATH |
| 产品图片 |
Sample:
Lane 1: Waxy corn (Plant) Lysate at 40 ug
Lane 2: Brassica napus (Plant) Lysate at 40 ug
Primary: Anti-Plant actin (bsm-33128M) at 1/1000 dilution
Secondary: IRDye800CW Goat Ant-Mouse IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 44 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
