产品中心-北京博奥森生物技术有限公司

产品编号	bs-10900R
英文名称	GAPDH Rabbit pAb, Loading Control
中文名称	3-磷酸甘油醛脱氢酶（内参）抗体
别名	38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.
	Specific References (53) \| bs-10900R has been referenced in 53 publications. [IF=8.101] Leyi Yao. et al. Plumbagin is a novel GPX4 protein degrader that induces apoptosis in hepatocellular carcinoma cells. FREE RADICAL BIO MED. 2023 Jul;203:1 WB ; Human. PubMed:37011699 [IF=7.917] Huang Shu. et al. Targeting nano-regulator based on metal–organic frameworks for enhanced immunotherapy of bone metastatic prostate cancer. CANCER NANOTECHNOL. 2023 Dec;14(1):1-15 WB ; Mouse,Human. PubMed:10.1186/s12645-023-00200-y [IF=7.74] Qifeng Deng . et al. Salmonella effector SpvB aggravates dysregulation of systemic iron metabolism via modulating the hepcidin−ferroportin axis. Gut Microbes. 2021;13(1):1-18 WB ; Mouse. PubMed:33475464 [IF=7.666] Valeria Lucchino. et al. Insights into the Genetic Profile of Two Siblings Affected by Unverricht-Lundborg Disease Using Patient-Derived hiPSCs. CELLS-BASEL. 2022 Jan;11(21):3491 WB ; Human. PubMed:36359887 [IF=6.832] Tao Cai. et al. Long noncoding RNA BBOX1-AS1 promotes the progression of gastric cancer by regulating the miR-361-3p/Mucin 13 signaling axis. BIOENGINEERED. 2022 Jun 05 WB ; Human. PubMed:10.1080/21655979.2022.2072629 [IF=6.706] Zhen-Xing Wang. et al. The Barrier-Enhancing Function of Soluble Yam (Dioscorea opposita Thunb.) Polysaccharides in Rat Intestinal Epithelial Cells, as Affected by the Covalent Se Conjugation. NUTRIENTS. 2022 Jan;14(19):3950 WB ; Rat. PubMed:36235602 [IF=6.34] Zhou et al. Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-?-mediated Nox4 expression. (2017) Redox.Biol. 11:390-402 WB ; Rat, Human. PubMed:28063381 [IF=6.17] Yinyun Ni. et al. miR-15a-5p inhibits metastasis and lipid metabolism by suppressing histone acetylation in lung cancer. Free Radical Bio Med. 2020 Dec;161:150 WB ; Human. PubMed:33059020 [IF=5.81] Zhou Z. et al. Vanillin Derivatives Reverse Fusobacterium nucleatum-Induced Proliferation and Migration of Colorectal Cancer Through E-Cadherin/β-Catenin Pathway.. Front Pharmacol. 2022 Mar;13:841918-841918 WB ; Human. PubMed:35308221 [IF=5.714] Zhaxi Mima. et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. INT IMMUNOPHARMACOL. 2022 Dec;113:109340 WB ; Mouse. PubMed:36330910 [IF=5.195] Yingjie Duan. et al. VX-765 ameliorates CKD VSMC calcification by regulating STAT3 activation. EUR J PHARMACOL. 2023 Apr;945:175610 WB ; Rat. PubMed:36858340 [IF=5.008] Liang et al. Itraconazole exerts its anti-melanoma effect by suppressing Hedgehog, Wnt, and PI3K/mTOR signaling pathways. (2017) Oncotarge. 8:28510-28525 WB ; Human. PubMed:28212537 [IF=5.008] Mu et al. Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/β-catenin signaling and is frequently methylated in breast cancer. (2017) Oncotarge. 8:39443-39459 WB ; Human. PubMed:28467796 [IF=4.784] Liu XQ et al. Sodium tanshinone IIA sulfonate protects against Aβ1–42-induced cellular toxicity by modulating Aβ-degrading enzymes in HT22 cells. Int J Biol Macromol. 2020 Feb 6;151:47-55. WB ; Mouse. PubMed:32035959 [IF=4.652] Lin et al. MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells. (2017) Cell.Physiol.Bioche. 41:124-136 WB ; Rat. PubMed:28214900 [IF=4.652] Zong et al. The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro. (2016) Cell.Physiol.Bioche. 38:1100-10 WB ; Mouse. PubMed:26938714 [IF=4.631] Shifei Li. et al. miR-31-5p Regulates Type I Interferon by Targeting SLC15A4 in Plasmacytoid Dendritic Cells of Systemic Lupus Erythematosus. J INFLAMM RES. 2022 Dec;15:6607-6616 WB ; Human. PubMed:36510495 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human. PubMed:26881030 [IF=4.486] Meiling Zhou. et al. Acupoint catgut embedding improves senescence in a rat model of ageing by regulating mitophagy via the PINK1 pathway. 2021 Mar 01 WB ; Rat. PubMed:33645011 [IF=4.302] Jia et al. Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α. (2018) J.Cell.Mol.Med. 22:4507-4521 WB ; Mouse. PubMed:29993193 [IF=4.268] Wu H et al. A UPLC-Q-TOF/MS-based plasma metabolomics approach reveals the mechanism of Compound Kushen Injection-based intervention against non-small cell lung cancer in Lewis tumor-bearing mice. Phytomedicine . 2020 Jun 2;76:153259. WB ; Mouse. PubMed:32534358 [IF=4.175] Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15 WB ; PubMed:33407505 [IF=4.175] Huajun Wang. et al. LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis. Cancer Cell Int. 2020 Dec;20(1):1-12 WB ; Human. PubMed:33292220 [IF=4.123] Sun L et al. Salmonella Effector SpvB Disrupts Intestinal Epithelial Barrier Integrity for Bacterial TranslocationFront Cell Infect Microbiol.2020 Dec 17;10:606541. WB ; Human. PubMed:33392110 [IF=3.647] Lin X et al. Sumoylation enhances the activity of the TGF-β/SMAD and HIF-1 signaling pathways in keloids. Life Sci . 2020 Aug 15;255:117859. WB ; Human. PubMed:32474020 [IF=3.641] Baozhen Qu. et al. Preserved antibacterial activity of ribosomal protein S15 during evolution. Mol Immunol. 2020 Nov;127:57 WB ; Shrimp, Amphioxus, Zebrafish and Mouse. PubMed:32927165 [IF=3.598] Chang L et al. Gypenoside A protects ischemia/reperfusion injuries by suppressing miR‐143‐3p level via the activation of AMPK/Foxo1 pathway. Biofactors. 2019 Dec 30. WB ; Rat. PubMed:31889343 [IF=3.558] Jie Cui. et al. A zinc finger family protein, ZNF263, promotes hepatocellular carcinoma resistance to apoptosis via activation of ER stress-dependent autophagy. Transl Oncol. 2020 Dec;13:100851 WB ; Human. PubMed:32898766 [IF=3.481] Jinzhu Han. et al. Circ_0027599 elevates RUNX1 expression via sponging miR-21-5p on gastric cancer progression. 2021 May 25 WB ; Human. PubMed:34032284 [IF=3.448] Wang T et al. Urotensin receptor antagonist urantide improves atherosclerosis-related kidney injury by inhibiting JAK2/STAT3 signaling pathway in rats. Life Sci. 2020 Feb 13;247:117421. WB ; Rat. PubMed:32061865
产品类型	内参抗体
研究领域	肿瘤细胞生物免疫学信号转导新陈代谢
抗体来源	Rabbit
克隆类型	Polyclonal
克隆号
交叉反应	Human,Mouse,Rat,Hamster
产品应用	WB=1:5000-50000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	38 kDa
检测分子量
细胞定位	细胞核细胞浆细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	Recombinant human GAPDH full length protein
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subunit: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS. Subcellular Location: Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions. Post-translational modifications: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated (Probable). Sulfhydration at Cys-152 increases catalytic activity. Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. SWISS: P04406 Gene ID: 2597 Database links: Entrez Gene: 374193 Chicken Entrez Gene: 2597 Human Entrez Gene: 100042025 Mouse Entrez Gene: 14433 Mouse Entrez Gene: 24383 Rat Entrez Gene: 685186 Rat Entrez Gene: 317743 Zebrafish Omim: 138400 Human SwissProt: P00356 Chicken SwissProt: P04406 Human SwissProt: P16858 Mouse SwissProt: P04797 Rat SwissProt: Q5XJ10 Zebrafish GAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。 GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。 GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.
产品图片	Sample: Lane 1,4,7: Mouse Cerebellum tissue lysates Lane 2,5,8: Rat Cerebellum tissue lysates Lane 3,6,9: Human SH-SY5Y cell lysates Primary: Anti-GAPDH (bs-10900R) at 1/10000~1/100000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 36 kDa Sample: Lane 1: Cerebellum (Mouse) Lysate Lane 2: Raw264.7 (Mouse) Cell Lysate Lane 3: NIH/3T3 (Mouse) Cell Lysate Lane 4: Cerebrum (Rat) Lysate Lane 5: SH-SY5Y (Human) Cell Lysate Lane 6: MCF-7 (Human) Cell Lysate Primary: Anti-GAPDH (bs-10900R) at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记