| 产品编号 | bs-11113R |
| 英文名称 | PCDH20 Rabbit pAb |
| 中文名称 | 原钙粘附蛋白20抗体 |
| 别 名 | PCD20_HUMAN; PCDH 13; PCDH 20; PCDH13; PCDH20; Protocadherin 13; Protocadherin 20; Protocadherin-13; Protocadherin-20; Protocadherin13; Protocadherin20. |
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Specific References (1) | bs-11113R has been referenced in 1 publications.
[IF=4.011] Hirose F et al. Expression of protocadherin-20 in mouse taste buds. Sci Rep. 2020 Feb 6;10(1):2051. IHF-F ; Mouse.
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| 研究领域 | 神经生物学 信号转导 细胞粘附分子 细胞骨架 细胞外基质 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCDH20: 611-710/924 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
As a subfamily of the cadherin superfamily, protocadherins are cadherin-like cell adhesion proteins that contain up to seven extracellular domains and are predominantly expressed in the nervous system. Importantly, the adhesion mechanism of protocadherins is distinct from classic cadherins. Through inactivation or overexpression, several protocadherins have been implicated in a variety of cancers. Protocadherin-20 (PCDH20), also known as protocadherin-13, is a 924 amino acid protein containing 6 cadherin domains and potentially functioning as a calcium-dependent cell-adhesion protein. In non-small cell lung cancer cell lines, a homozygous loss of PCDH20 was identified through either deletion of one allele and methylation of the other or methylation of both alleles. Hypermethylation of PCDH20 is associated with worse prognosis and clinical outcome, suggesting that PCDH20 may function as a tumor suppressor. Function: Potential calcium-dependent cell-adhesion protein. Subcellular Location: Cell membrane. Similarity: Contains 6 cadherin domains. SWISS: Q8N6Y1 Gene ID: 64881 Database links: Entrez Gene: 64881 Human Omim: 614449 Human SwissProt: Q8N6Y1 Human Unigene: 391781 Human |
| 产品图片 |
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human U87MG cell lysates
Primary: Anti-PCDH20 (bs-11113R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 102 kDa
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Olfactory bulb (Mouse) Lysate at 40 ug
Primary: Anti-PCDH20 (bs-11113R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 100 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SV2B) Polyclonal Antibody, Unconjugated (bs-11113R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- PCDH20 antibody(bs-11113R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
