| 产品编号 | bs-0369M-HRP |
| 英文名称 | Mouse Anti-Rabbit IgM, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记的小鼠抗兔IgM |
| 别 名 | Mouse Anti-Rabbit IgM (HRP); Immunoglobulin M; |
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Specific References (2) | bs-0369M-HRP has been referenced in 2 publications.
[IF=2.35] Hou, Hong‑Wei, et al. "Triptolide exerts protective effects against fibrosis following ileocolonic anastomosis by mechanisms involving the miR-16-1/HSP70 pathway in IL-10-deficient mice." International Journal of Molecular Medicine. WB ; Rabbit.
[IF=1.922] Cao et al. Dickkopf‑3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. (2018) Mol.Med.Rep. 17:7249-7257 WB ; Rabbit.
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| 抗体来源 | Mouse |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rabbit |
| 产品应用 | WB=1:1000-10000,IHC-P=1:100-500,IHC-F=1:100-1000,ELISA=1:1000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 2.0 mg/ml |
| 免 疫 原 | Native Rabbit IgM |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Immunoglobulin M (IgM) normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
