产品中心-北京博奥森生物技术有限公司

产品编号	bs-0287R
英文名称	His tag Rabbit pAb
中文名称	His tag标签抗体
别名	HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
	Specific References (17) \| bs-0287R has been referenced in 17 publications. [IF=2.914] WB ; Pig. PubMed:32649964 [IF=19.924] Yifan Yang. et al. A Versatile Platform for the Tumor-Targeted Intracellular Delivery of Peptides, Proteins, and siRNA. ADV FUNCT MATER. 2023 Apr;:2301011 WB ; Human. PubMed:10.1002/adfm.202301011 [IF=7.2] Zhiyan Hu. et al. CIP4 targeted to recruit GTP-Cdc42 involving in invadopodia formation via NF-κB signaling pathway promotes invasion and metastasis of CRC. Mol Ther-Oncolytics. 2022 Feb;: WB ; Strain（Bl21）. PubMed:10.1016/j.omto.2022.02.023 [IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126. IHC-P ; PubMed:23198829 [IF=4.235] Xu Zhenshang. et al. Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases. Front Microbiol. 2020 Nov;11:2281 WB ; PubMed:33329424 [IF=3.309] Chuandong Cheng. et al. SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma. PATHOL RES PRACT. 2022 Oct;:154176 WB ; Human. PubMed:36327817 [IF=2.984] Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene . 2020 Oct 30;759:144999. WB ; pig. PubMed:32717305 [IF=2.984] WB, IF ; Pig. PubMed:32717305 [IF=2.914] Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379 WB ; Pig. PubMed:32649964 [IF=2.913] Li N et al. Identification and characterization of an atypical RIG-I encoded by planarian Dugesia japonica and its essential role in the immune response.(2019) Dev Comp Immunol. Feb;91:72-84. WB ; PubMed:30355517 [IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013). WB ; PubMed:Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gen [IF=2.7] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012). PubMed:Life Science Journal 9.1 (2012) [IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969 WB ; PubMed:ISSN:1097-8135 [IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013). WB ; PubMed:23774531 [IF=1.676] Ma et al. Adjuvant effects mediated by the carbohydrate recognition domain of Agrocybe aegerita lectin interacting with avian influenza H9N2 viral surface glycosylated proteins. (2017) J.Zhejiang.Univ.Sci.. 18:653-661 other ; PubMed:28786240 [IF=1.109] He,et al.Recombinant Fiber-2 Protein-Based Indirect ELISA for Antibody Detection of Fowl Adenovirus Serotype 4.(2018) Avian Diseases. 62:73-78. WB ; PubMed:29620471 [IF=0] Ren et al. Long-term rescue of rat retinal ganglion cells and visual function by AAV-mediated BDNF expression after acute elevation of intraocular pressure. (2012) Invest.Ophthalmol.Vis.Sci. 53:1003-11 IHC ; PubMed:22247466
产品类型	标签抗体
研究领域	肿瘤细胞生物免疫学染色质和核信号信号转导合成与降解结合蛋白
抗体来源	Rabbit
克隆类型	Polyclonal
克隆号
交叉反应	Recombinant protein
产品应用	WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	kDa
检测分子量
性状	Liquid
浓度	1mg/ml
免疫原	Synthetic peptide: HHHHHH conjugated to KLH
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. 标签抗体蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。 2）可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括：细菌，杆状病毒和哺乳动物） 4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌 5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫
产品图片	20 ng rGFP-His protein (bs-33009P) per lane probed with His tag polyclonal antibody respectively, unconjugated (bs-0287R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记